Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica
학술지
PloS one
저자명
Wang, Wei; Wei, Hui; Alahuhta, Markus; Chen, Xiaowen; Hyman, Deborah; Johnson, David K.; Zhang, Min; Himmel, Michael E.
초록
<P>To develop a direct microbial sugar conversion platform for the production of lipids, drop-in fuels and chemicals from cellulosic biomass substrate, we chose <I>Yarrowia lipolytica</I> as a viable demonstration strain. <I>Y. lipolytica</I> is known to accumulate lipids intracellularly and is capable of metabolizing sugars to produce lipids; however, it lacks the lignocellulose-degrading enzymes needed to break down biomass directly. While research is continuing on the development of a <I>Y. lipolytica</I> strain able to degrade cellulose, in this study, we present successful expression of several xylanases in <I>Y. lipolytica</I>. The XynII and XlnD expressing <I>Yarrowia</I> strains exhibited an ability to grow on xylan mineral plates. This was shown by Congo Red staining of halo zones on xylan mineral plates. Enzymatic activity tests further demonstrated active expression of XynII and XlnD in <I>Y. lipolytica</I>. Furthermore, synergistic action in converting xylan to xylose was observed when XlnD acted in concert with XynII. The successful expression of these xylanases in <I>Yarrowia</I> further advances us toward our goal to develop a direct microbial conversion process using this organism.</P>