초록
<P><B>Abstract</B></P><P>A fermentative hydrogen-producing strain, RZF-1108, was isolated from a biohydrogen reactor, and identified as <I>Clostridium beijerinckii</I> on the basis of the 16S rRNA gene analysis and physiobiochemical characteristics<I>.</I> The effects of culture conditions on hydrogen production by <I>C. beijerinckii</I> RZF-1108 were investigated in batch cultures. The hydrogen production and growth of strain RZF-1108 were highly dependent on temperature, initial pH and substrate concentration. Yeast extract was a favorable nitrogen source for hydrogen production and growth of RZF-1108. Hydrogen production corresponded to cell biomass yield in different culture conditions. The maximum hydrogen evolution, yield and production rate of 2209ml H<SUB>2</SUB>/l medium, 1.97mol H<SUB>2</SUB>/mol glucose and 104.20ml H<SUB>2</SUB>/g CDWh<SUP>−1</SUP> were obtained at 9g/l of glucose, initial pH of 7.0, inoculum volume of 8% and temperature of 35°C, respectively. These results demonstrate that <I>C. beijerinckii</I> can efficiently produce H<SUB>2</SUB>, and is another model microorganism for biohydrogen investigations.</P>