BioChem - DOS

Immobilization of Lactobacillus rhamnosus in mesoporous silica-based material: An efficiency continuous cell-recycle fermentation system for lactic acid production

Journal of bioscience and bioengineering

Zhao, Z.; Xie, X.; Wang, Z.; Tao, Y.; Niu, X.; Huang, X.; Liu, L.; Li, Z.

Lactic acid bacteria immobilization methods have been widely used for lactic acid production. Until now, the most common immobilization matrix used is calcium alginate. However, Ca-alginate gel disintegrated during lactic acid fermentation. To overcome this deficiency, we developed an immobilization method in which Lactobacillus rhamnosus cells were successfully encapsulated into an ordered mesoporous silica-based material under mild conditions with a high immobilization efficiency of 78.77% by using elemental analysis. We also optimized the cultivation conditions of the immobilized L. rhamnosus and obtained a high glucose conversion yield of 92.4%. Furthermore, L. rhamnosus encapsulated in mesoporous silica-based material exhibited operational stability during repeated fermentation processes and no decrease in lactic acid production up to 8 repeated batches.

2016

Society for Bioscience and Bioengineering, Japan ; Distributed outside Japan by Elsevier Science

1389-1723

1347-4421

121

6

pp.645-651

10.1016/j.jbiosc.2015.11.010

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75437199

Lactic acid production; Mesoporous silica-based material; Lactobacillus rhamnosus; Cell immobilization; Ca-alginate