초록
<P><B>Abstract</B></P> <P>A potential commercial pretreatment for furfural residues (FRs) was investigated by using a combination of green liquor and hydrogen peroxide (GL–H<SUB>2</SUB>O<SUB>2</SUB>). The results showed that 56.2% of lignin removal was achieved when the sample was treated with 0.6g H<SUB>2</SUB>O<SUB>2</SUB>/g-DS (dry substrate) and 6mL GL/g-DS at 80°C for 3h. After 96h hydrolysis with 18FPU/g-cellulose for cellulase, 27CBU/g-cellulose for β-glucosidase, the glucose yield increased from 71.2% to 83.6%. Ethylenediaminetetraacetic acid was used to reduce the degradation of H<SUB>2</SUB>O<SUB>2</SUB>, the glucose yield increased to 90.4% after the addition of 1% (w/w). The untreated FRs could bind more easily to cellulase than pretreated FRs could. The structural changes on the surface of sample were characterized by X-ray photoelectron spectroscopy. The results indicated that the surface lignin could be effectively removed during pretreatment, thereby decreasing the enzyme–lignin binding activity. Moreover, the carbonyl from lignin plays an important role in cellulase binding.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Green liquor coupled with peroxide (GL–H<SUB>2</SUB>O<SUB>2</SUB>) removed lignin effectively. </LI> <LI> Glucose yield was greatly improved after GL–H<SUB>2</SUB>O<SUB>2</SUB> pretreatment. </LI> <LI> The loading of H<SUB>2</SUB>O<SUB>2</SUB> can be decreased by adding ethylenediaminetetraacetic acid. </LI> <LI> Combined pretreatment removes the lignin and reduces the cellulase–lignin binding. </LI> <LI> The carbonyl from lignin plays an important role in cellulase binding. </LI> </UL> </P>