BioChem - DOS

Integrated production of gluconic acid and xylonic acid using dilute acid pretreated corn stover by two-stage fermentation

Biochemical engineering journal

Zhou, Xuelian; Zhou, Xin; Liu, Guang; Xu, Yong; Balan, Venkatesh

<P><B>Abstract</B></P> <P>A sustainable and efficient two-stage fermentation was developed to produce gluconic acid (GA) and xylonic acid (XA) from dilute acid pretreated corn stover (DA-CS) using <I>Gluconobacter oxydans.</I> Cells (6.2 g/L) were obtained after four cycles of fermentation in DA-CS enzyme hydrolysate at 50 ml scale in a shake flask. With each cycle showing complete utilization of glucose with GA productivity rate at 5.3 g/L/h. The enriched cells were then collected and used to ferment DA-CS liquid stream (generated during pretreatment containing mostly xylose) at 50 ml scale in a shake flask to produce XA at a rate of 1.9 g/L/h. Subsequently, the process was scaled up to 1 L. Both GA and XA was produced at the rate of 8.7 g/L/h and 3.7 g/L/h respectively in 36 h. From 1 kg of corn stover we were able to produce 296.2 g GA and 167.4 g XA by two stage fermentation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An efficient and sustainable two-stage fermentation was developed using <I>G. oxydans</I>. </LI> <LI> Bacterial cells enriched during GA production was recycled for XA production. </LI> <LI> High productivity of 8.66 g/L/h GA and 3.69 g/L/h XA was achieved in COS-SSTR. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2018

Elsevier

1369-703x

137

pp.18-22

10.1016/j.bej.2018.05.005

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART86698895

Corn stover; Gluconic acid; Xylonic acid; Gluconobacter oxydans; Two-stage fermentation; Dilute acid pretreatment