BioChem - DOS

Optimization of fermentative hydrogen production from hydrolysate of microwave assisted sulfuric acid pretreated oil palm trunk by hot spring enriched culture

International journal of hydrogen energy

Khamtib, S.; Plangklang, P.; Reungsang, A.

The optimum condition for hydrolysis of oil palm trunk (OPT) by microwave-H<SUB>2</SUB>SO<SUB>4</SUB> method and hydrogen production from OPT hydrolysate were explored using response surface methodology (RSM) with central composite design (CCD). The optimum condition for hydrolysis of OPT was 1.56% (w/v) H<SUB>2</SUB>SO<SUB>4</SUB> and 7.50 min reaction time at 450 W. At this condition, 8.95+/-0.99 g/L glucose/L, 8.29+/-0.43 g/L xylose, 4.57+/-0.14 g/L arabinose and 2.61+/-0.11 g/L acetic were obtained. The individual and interactive effects of the initial pH, initial substrate concentration and NaHCO<SUB>3</SUB> concentration on the hydrogen production potential (P<SUB>s</SUB>) from OPT hydrolysate by hot spring enriched culture were further investigated using RSM with CCD. The initial pH and initial substrate concentration had a statistically individual effect on P<SUB>s</SUB>, while the interactive effect on P<SUB>s</SUB> was found between the initial pH and initial substrate concentration. The optimum condition obtained was an initial pH of 6.03, an initial substrate concentration of 22.07 g/L and a NaHCO<SUB>3</SUB> concentration of 6.71 g/L in which a P<SUB>s</SUB> of 1947 mL H<SUB>2</SUB>/L medium was achieved. Microbial community analysis by PCR-DGGE indicated that Clostridium sp. and Lactobacillus sp. were the predominant species at the optimum condition.

2011

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

36

21

pp.14204-14216

10.1016/j.ijhydene.2011.05.117

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57433434

Thermophilic; Bio-hydrogen; Oil palm trunk; Microwave-H2SO4 pretreatment; Response surface methodology; Hydrolysate