초록
The effectiveness of several pretreatments [high-power ultrasound, sulfuric acid (H<SUB>2</SUB>SO<SUB>4</SUB>), sodium hydroxide (NaOH), and ammonium hydroxide (NH<SUB>3</SUB>OH)] to enhance glucose production from insoluble fractions recovered from enzyme-assisted aqueous extraction processing of extruded full-fat soybean flakes (FFSF) was investigated. Sonication of the insoluble fraction at 144μm<SUB>pp (peak-to-peak)</SUB> for 30 and 60s did not improve the saccharification yield. The solid fractions recovered after pretreatment with H<SUB>2</SUB>SO<SUB>4</SUB> [1% (w/w), 90<SUP>o</SUP>C, 1.5h], NaOH [15% (w/w), 65<SUP>o</SUP>C, 17h], and NH<SUB>3</SUB>OH [15% (w/w), 65<SUP>o</SUP>C, 17h] showed significant lignin degradation, i.e., 81.9%, 71.2%, and 75.4%, respectively, when compared to the control (7.4%). NH<SUB>3</SUB>OH pretreatment resulted in the highest saccharification yield (63%) after 48h of enzymatic saccharification. A treatment combining the extraction and saccharification steps and applied directly to the extruded FFSF, where oil extraction yield and saccharification yield reached 98% and 43%, respectively, was identified.