Two-stage transcriptional reprogramming in Saccharomyces cerevisiae for optimizing ethanol production from xylose
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
기타
바이오정밀화학
용매
화학제품
연료
기타
화장품용 기능성소재
계면활성제⁄증점제
기타
의료용 화학소재
식품첨가제
논문
Two-stage transcriptional reprogramming in Saccharomyces cerevisiae for optimizing ethanol production from xylose
학술지
Metabolic engineering
저자명
Cao, L.; Tang, X.; Zhang, X.; Zhang, J.; Tian, X.; Wang, J.; Xiong, M.; Xiao, W.
초록
Conversion of lignocellulosic material to ethanol is a major challenge in second generation bio-fuel production by yeast Saccharomyces cerevisiae. This report describes a novel strategy named ''two-stage transcriptional reprogramming (TSTR)'' in which key gene expression at both glucose and xylose fermentation phases is optimized in engineered S. cerevisiae. Through a combined genome-wide screening of stage-specific promoters and the balancing of the metabolic flux, ethanol yields and productivity from mixed sugars were significantly improved. In a medium containing 50g/L glucose and 50g/L xylose, the top-performing strain WXY12 rapidly consumed glucose within 12h and within 84h it consistently achieved an ethanol yield of 0.48g/g total sugar, which was 94% of the theoretical yield. WXY12 utilizes a KGD1 inducible promoter to drive xylose metabolism, resulting in much higher ethanol yield than a reference strain using a strong constitutive PGK1 promoter. These promising results validate the TSTR strategy by synthetically regulating the xylose assimilation pathway towards efficient xylose fermentation.