Marker removal system for Thermoanaerobacterium saccharolyticum and development of a markerless ethanologen
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
기타
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
치료제
식품첨가제
논문
Marker removal system for Thermoanaerobacterium saccharolyticum and development of a markerless ethanologen
학술지
Applied and environmental microbiology
저자명
Shaw, A. Joe; Covalla, Sean F.; Hogsett, David A.; Herring, Christopher D.
초록
<P><B>ABSTRACT</B><P> Marker removal strategies were developed for <I>Thermoanaerobacterium saccharolyticum</I> to select against the <I>pyrF</I> gene and the <I>pta</I> and <I>ack</I> genes. The <I>pta</I> - and <I>ack</I> -based haloacetate selective strategy was subsequently used to create strain M0355, a markerless Δ <I>ldh</I> Δ <I>pta</I> Δ <I>ack</I> strain that produces ethanol at a high yield. </P></P>