BioChem - DOS

Succinic acid production and CO2 fixation using a metabolically engineered Escherichia coli in a bioreactor equipped with a self-inducing agitator

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Wu, Hui; Li, Qing; Li, Zhi-min; Ye, Qin

<P><B>Highlights</B></P><P>&#x025BA; A reactor equipped with a self-inducing agitator was used to recycle CO<SUB>2</SUB> for succinic acid production. &#x025BA; The self-inducing agitator reduced CO<SUB>2</SUB> waste. &#x025BA; At pH6.3 less CO<SUB>2</SUB> was required but succinate production was not affected. &#x025BA; This process was coupled with ethanol fermentation to reduce CO<SUB>2</SUB> emission.</P> <P><B>Abstract</B></P><P>A 5-L bioreactor equipped with a self-induction agitator was applied to a two-stage culture of <I>Escherichia coli</I> NZN111 for succinic acid production in a mineral salts medium. CO<SUB>2</SUB> was cycled inside this reactor and a sufficient CO<SUB>2</SUB> transfer rate was maintained with the elimination of CO<SUB>2</SUB> wasted by ventilation. In the anaerobic stage, much less supplemental CO<SUB>2</SUB> was required at pH6.3 compared to that at pH7.0, and the succinate yield increased. The performances of succinate production were little changed when compared to a process with CO<SUB>2</SUB> sparging indicating that use of the self-inducing agitator reduced CO<SUB>2</SUB> waste. The succinate production process was further coupled with ethanol fermentation by using the CO<SUB>2</SUB> produced from ethanol fermentation. This integrated system demonstrated that both succinate and bioethanol can be effectively produced while the emission of the CO<SUB>2</SUB> formed during ethanol fermentation can be greatly reduced.</P>

2012

0960-8524

107

pp.376-384

10.1016/j.biortech.2011.12.043

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58443379

Escherichia coli; Succinic acid; CO2 fixation; Self-induction agitator; Bioreactor