초록
In this study, a fibrous bed bioreactor (FBB) was used for <TEX>$\small{D}$</TEX>-lactic acid (<TEX>$\small{D}$</TEX>-LA) production by Sporolactobacillus inulinus Y2-8. Corn flour hydrolyzed with <TEX>${\alpha}$</TEX>-amylase and saccharifying enzyme was used as a cost-efficient and nutrient-rich substrate for <TEX>$\small{D}$</TEX>-LA production. A maximal starch conversion rate of 93.78% was obtained. The optimum pH for <TEX>$\small{D}$</TEX>-LA production was determined to be 6.5. Ammonia water was determined to be an ideal neutralizing agent, which improved the <TEX>$\small{D}$</TEX>-LA production and purification processes. Batch fermentation and fed-batch fermentation, with both free cells and immobilized cells, were compared to highlight the advantages of FBB fermentation. In batch mode, the <TEX>$\small{D}$</TEX>-LA production rate of FBB fermentation was 1.62 g/l/h, which was 37.29% higher than that of free-cell fermentation, and the <TEX>$\small{D}$</TEX>-LA optical purities of the two fermentation methods were above 99.00%. In fe<TEX>$\small{D}$</TEX>-batch mode, the maximum <TEX>$\small{D}$</TEX>-LA concentration attained by FBB fermentation was 218.8 g/l, which was 37.67% higher than that of free-cell fermentation. Repeate<TEX>$\small{D}$</TEX>-batch fermentation was performed to determine the long-term performance of the FBB system, and the data indicated that the average <TEX>$\small{D}$</TEX>-LA production rate was 1.62 g/l/h and the average yield was 0.98 g/g. Thus, hydrolyzed corn flour fermented by S. inulinus Y2-8 in a FBB may be used for improving <TEX>$\small{D}$</TEX>-LA fermentation by using ammonia water as the neutralizing agent.