<P>Bacterial cellulose (BC) is widely used in industries owing to its high purity and strength. Although <I>Komagataeibacter nataicola</I> is a representative species for BC production, its intracellular metabolism leading to BC secretion is unclear. In the present study, a genome-scale metabolic network of cellulose-producing <I>K. nataicola</I> strain RZS01 was reconstructed to understand its metabolic behavior. This model <I>i</I>HZ771 comprised 771 genes, 2035 metabolites, and 2014 reactions. Constraint-based analysis was used to characterize and evaluate the critical intracellular pathways. The analysis revealed that a total of 71 and 30 genes are necessary for cellular growth in a minimal medium and complex medium, respectively. Glycerol was identified as the optimal carbon source for the highest BC production. The minimization of metabolic adjustment algorithm identified 8 genes as potential targets for over-production of BC. Overall, model <I>i</I>HZ771 proved to be a useful platform for understanding the physiology and BC production of <I>K. nataicola</I>.</P>