초록
The operational conditions of H<SUB>2</SUB> production from benzoate by Rhodopseudomonas palustris was optimized in batch experiments by using the response surface methodology (RSM) and a desirability function approach. The estimated best H<SUB>2</SUB> production performance, i.e., maximum substrate-to-hydrogen conversion efficiency of 0.61 and light conversion efficiency of 3.20%, was achieved at: benzoate concentration 9.97 mM, NH<SUB>4</SUB>Cl concentration 0.21 g/L and pH 6.76. These parameters were employed for running a continuous H<SUB>2</SUB> production bioreactor, for which the optimal hydraulic retention time was found to be 6 d. Under a light-dark cycle, R. palustris survived and maintained their H<SUB>2</SUB> production activity in the continuous operating system. The results demonstrate that it is possible to operate an outdoor photo-bioreacror for continuous H<SUB>2</SUB> production by utilizing aromatic compound as substrate. This works offer implications for guiding the design and operation of more energy-productive processes for treatment of aromatic compound-containing wastewater treatment.