초록
Bio-hydrogen production from sugarcane vinasse in anaerobic up-flow packed-bed reactors (APBR) was evaluated. Four types of support materials, expanded clay (EC), charcoal (Ch), porous ceramic (PC), and low-density polyethylene (LDP) were tested as support for biomass attachment. APBR (working volume - 2.3 L) were operated in parallel at a hydraulic retention time of 24 h, an organic loading rate of 36.2 kg-COD m<SUP>-3</SUP> d<SUP>-1</SUP>, at 25 <SUP>o</SUP>C. Maximum volumetric hydrogen production values of 509.5, 404, 81.4 and 10.3 mL-H<SUB>2</SUB> d<SUP>-1</SUP> L<SUP>-1</SUP><SUB>reactor</SUB> and maximum yields of 3.2, 2.6, 0.4 and 0.05 mol-H<SUB>2</SUB> mol<SUP>-1</SUP> carbohydrates total, were observed during the monitoring of the reactors filled with LDP, EC, Ch and PC, respectively. Thus, indicating the strong influence of the support material on H<SUB>2</SUB> production. LDP was the most appropriate material for hydrogen production among the materials evaluated. 16S rRNA gene by Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis and scanning electron microscopy confirmed the selection of different microbial populations. 454-pyrosequencing performed on samples from APBR filled with LDP revealed the presence of hydrogen-producing organisms (Clostridium and Pectinatus), lactic acid bacteria and non-fermentative organisms.