Methanogenic capacity and robustness of hydrogenotrophic cultures based on closed nutrient recycling via microbial catabolism: Impact of temperature and microbial attachment
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Methanogenic capacity and robustness of hydrogenotrophic cultures based on closed nutrient recycling via microbial catabolism: Impact of temperature and microbial attachment
<P><B>Abstract</B></P> <P>A biological methanation system based on nutrient recycling via mixed culture microbial catabolism was investigated at mesophilic (37 °C) and thermophilic (55 °C) temperatures. At mesophilic temperatures, the formation of biofilms on two different types of material was assessed. Results showed that with intense mixing the biofilm reactors presented methanogenic capacities (per working volume) 50% higher than the ones operated with suspended cultures. Gas feeding rates of 200 L/L/d were achieved at a H<SUB>2</SUB>/CO<SUB>2</SUB> to CH<SUB>4</SUB> conversion efficiency of above 90% by linking two reactors in series. Furthermore the robustness of the cultures was assessed under a series of inhibitory conditions that simulated possible process interferences at full scale operation. Full recovery after separate intense oxygenation and long starvation periods was observed within 2–5 days.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enrichment of hydrogenotrophic cultures was performed at 37 °C and 55 °C. </LI> <LI> Hydrogenotrophic biofilms were allowed to develop on two different support media. </LI> <LI> Recovery rates after oxygenation were better at mesophilic temperatures. </LI> <LI> Recovery after starvation and oxygenation was complete after 3–5 days. </LI> <LI> Biofilms can be formed under intense agitation to help increase methanogenic capacity. </LI> </UL> </P>