초록
A Box-Behnken design of response surface method was used to optimize acetic acid-catalyzed hydrothermal pretreatment of corn stover, in respect to acid concentration (0.05-0.25%), treatment time (5-15min) and reaction temperature (180-210<SUP>o</SUP>C). Acidogenic fermentations with different initial pH and hydrolyzates were also measured to evaluate the optimal pretreatment conditions for maximizing acid production. The results showed that pretreatment with 0.25% acetic acid at 191<SUP>o</SUP>C for 7.74min was found to be the most optimal condition for pretreatment of corn stover under which the production of acids can reach the highest level. Acidogenic fermentation with the hydrolyzate of pretreatment at the optimal condition at the initial pH=5 was shown to be butyric acid type fermentation, producing 21.84g acetic acid, 7.246g propionic acid, 9.170 butyric acid and 1.035g isovaleric acid from 100g of corn stover in 900g of water containing 2.25g acetic acid.