BioChem - DOS

Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Saratale, Rijuta Ganesh; Shin, Han Seung; Ghodake, Gajanan S.; Kumar, Gopalakrishnan; Oh, Min Kyu; Saratale, Ganesh Dattatraya

<P><B>Abstract</B></P> <P>This study focuses on development of calcium peroxide (CaO<SUB>2</SUB>) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO<SUB>4</SUB>) and cobalt chloride (COCl<SUB>2</SUB>) in CaO<SUB>2</SUB> pretreatment resulted in maximum delignification of KCP relative to individual CaO<SUB>2</SUB> pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain <I>Klebsiella pneumoniae</I> KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Developed CaO<SUB>2</SUB> pretreatment &amp; optimized various operational parameters for KCP biomass. </LI> <LI> Addition of inorganic salts improved delignification, glucose yield and total sugar recovery. </LI> <LI> Maximum BDO production (10.42 g/L) was achieved using <I>Klebsiella pneumoniae</I> KMK05. </LI> <LI> This novel pretreatment strategy abstained loss of sugar components of biomass. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2018

Elsevier

0960-8524

258

pp.26-32

10.1016/j.biortech.2018.02.115

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART84775040

Kenaf core powder; Calcium peroxide pretreatment; Enzymatic hydrolysis; Klebsiella pneumoniae KMK05; 2,3-Butanediol production