BioChem - DOS

Isolation of a euryhaline microalgal strain, Tetraselmis sp. CTP4, as a robust feedstock for biodiesel production

Scientific reports

Pereira, Hugo; Gangadhar, Katkam N.; Schulze, Peter S. C.; Santos, Tamá ra; de Sousa, Carolina Bruno; Schueler, Lisa M.; Custó dio, Luí sa; Malcata, F. Xavier; Gouveia, Luí sa; Varela, Joã o C. S.; Barreira, Luí sa

<P>Bioprospecting for novel microalgal strains is key to improving the feasibility of microalgae-derived biodiesel production. <I>Tetraselmis</I> sp. CTP4 (Chlorophyta, Chlorodendrophyceae) was isolated using fluorescence activated cell sorting (FACS) in order to screen novel lipid-rich microalgae. CTP4 is a robust, euryhaline strain able to grow in seawater growth medium as well as in non-sterile urban wastewater. Because of its large cell size (9&#x2013;22 &mu;m), CTP4 settles down after a six-hour sedimentation step. This leads to a medium removal efficiency of 80%, allowing a significant decrease of biomass dewatering costs. Using a two-stage system, a 3-fold increase in lipid content (up to 33% of DW) and a 2-fold enhancement in lipid productivity (up to 52.1 mg L<SUP>&#x2212;1</SUP> d<SUP>&#x2212;1</SUP>) were observed upon exposure to nutrient depletion for 7 days. The biodiesel synthesized from the lipids of CTP4 contained high levels of oleic acid (25.67% of total fatty acids content) and minor amounts of polyunsaturated fatty acids with ≥4 double bonds (<1%). As a result, this biofuel complies with most of the European (EN14214) and American (ASTM D6751) specifications, which commonly used microalgal feedstocks are usually unable to meet. In conclusion, <I>Tetraselmis</I> sp. CTP4 displays promising features as feedstock with lower downstream processing costs for biomass dewatering and biodiesel refining.</P>

2016

Nature Publishing Group

cc-by

2045-2322

6

pp.35663

10.1038/srep35663

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART76691307

https://www.nature.com/articles/srep35663.pdf