BioChem - DOS

The synthesis of n-caproate from lactate: a new efficient process for medium-chain carboxylates production

Scientific reports

Zhu, Xiaoyu; Tao, Yong; Liang, Cheng; Li, Xiangzhen; Wei, Na; Zhang, Wenjie; Zhou, Yan; Yang, Yanfei; Bo, Tao

<P>A unique microbiome that metabolizes lactate rather than ethanol for <I>n</I>-caproate production was obtained from a fermentation pit used for the production of Chinese strong-flavour liquor (CSFL). The microbiome was able to produce <I>n</I>-caproate at concentrations as high as 23.41 g/L at a maximum rate of 2.97 g/L/d in batch trials without in-line extraction. Compared with previous work using ethanol as the electron donor, the <I>n</I>-caproate concentration increased by 82.89%. High-throughput sequencing analysis showed that the microbiome was dominated by a <I>Clostridium</I> cluster IV, which accounted for 79.07% of total reads. A new process for <I>n</I>-caproate production was proposed, lactate oxidation coupled to chain elongation, which revealed new insight into the well-studied lactate conversion and carbon chain elongation. In addition, these findings indicated a new synthesis mechanism of <I>n</I>-caproate in CSFL. We believe that this efficient process will provide a promising opportunity for the innovation of waste recovery as well as for <I>n</I>-caproate biosynthesis.</P>

2015

Nature Publishing Group

cc-by

2045-2322

5

pp.14360

10.1038/srep14360

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART73139295

https://www.nature.com/articles/srep14360.pdf