Use of a mannitol rich ensiled grass press juice (EGPJ) as a sole carbon source for polyhydroxyalkanoates (PHAs) production through high cell density cultivation

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논문

Use of a mannitol rich ensiled grass press juice (EGPJ) as a sole carbon source for polyhydroxyalkanoates (PHAs) production through high cell density cultivation

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Cerrone, F.; Davis, R.; Kenny, S.T.; Woods, T.; O'Donovan, A.; Gupta, V.K.; Tuohy, M.; Babu, R.P.; O'Kiely, P.; O'Connor, K.

초록

This study demonstrates the use of a mannitol rich ensiled grass press juice (EGPJ) as a renewable carbon substrate for polyhydroxyalkanoates (PHA) production in shaking flask experiments and fed-batch stirred tank reactor cultivations. Fed-batch cultivations of Burkholderia sacchari IPT101 using EGPJ as sole carbon source produced 44.5g/L CDW containing 33% polyhydroxybutyrate (PHB) in 36h, while Pseudomonas chlororaphis IMD555 produced a CDW of 37g/L containing 10% of medium chain length polyhydroxyalkanoates (mcl-PHA) in 34h. PHB and mcl-PHA extracted from B. sacchari IPT101 and P. chlororaphis IMD555, grown on EGPJ, had a molecular weight of 548kg/mol and 115.4kg/mol, respectively. While mcl-PHA can be produced from EGPJ, PHB production is more interesting as there is a 4-fold higher volumetric productivity compared to mcl-PHA.

발행연도

2015

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

191

페이지

pp.45-52

DOI

10.1016/j.biortech.2015.04.128