BioChem - DOS

An Ustilago maydis chassis for itaconic acid production without by-products

Microbial biotechnology

Becker, Johanna; Hosseinpour Tehrani, Hamed; Gauert, Marc; Mampel, Jö rg; Blank, Lars M.; Wierckx, Nick

<P><B>Summary</B></P><P><I>Ustilago maydis</I> is a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild&#x2010;type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by&#x2010;product spectrum of <I>U.&nbsp;maydis</I> was reduced through strain engineering using CRISPR/Cas9 and FLP/FRT, greatly increasing the metabolic flux into the targeted itaconate biosynthesis pathway. With this strategy, a marker&#x2010;free chassis strain could be engineered, which produces itaconate from glucose with significantly enhanced titre, rate and yield. The lack of by&#x2010;product formation not only benefited itaconate production, it also increases the efficiency of downstream processing improving cell handling and product purity.</P>

2020

John Wiley and Sons Inc.

1751-7907

1751-7915

13

2

pp.350-362

10.1111/1751-7915.13525

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART101795410