초록
<P><B>Abstract</B></P> <P>This study is aimed to improving extracellular lipase production by <I>Trichoderma</I> strains using oil palm empty fruit bunch (OPEFB) medium on solid state fermentation with the assistance of statistical optimization. Two selected <I>Trichoderma</I> strains (<I>Trichoderma</I> sp.1 and <I>Hypocrea neorufa.1</I>) with high lipase producing potential were employed in a stepwise optimization. Initially, the influence of nutritional (carbon, nitrogen sources, and inducers) and environmental (moisture and temperature) factors on lipase production was evaluated using One-Factor-At-A-time (OFAT) method. Next, three most influential factors (Glucose, Urea and Olive oil) resulting from OFAT were optimized for lipase production by <I>Trichoderma</I> sp.1 using the central composite design under response surface methodology. Experiments conducted under optimum conditions (10.38% (w/w) glucose, 0.86% (w/w) urea, and 7.38% (v/w) olive oil) were further improved the lipase production from 0.195 to 0.390Ug<SUP>−1</SUP>, which is about 2 folds greater than un-optimized condition. This study demonstrated that OPEFB can be utilized as inexpensive substrate for improving the lipase secretion by <I>Trichoderma</I> sp.1.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lipase production using oil-palm fruit-bunch medium in solid state fermentation. </LI> <LI> <I>Trichoderma</I> strains with higher lipase producing potential were employed. </LI> <LI> Parameters on lipase production were investigated by One-Factor-At-A-time method. </LI> <LI> Three most influential parameters resulting from OFAT were optimized. </LI> <LI> Improved the lipase production is ∼2 folds greater. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>