BioChem - DOS

Production and properties of lipase of Aeromonas sobria

Microbiology and immunology

Takahashi, Eizo; Ito, Hidenobu; Kobayashi, Hidetomo; Yamanaka, Hiroyasu; Takeda, Yoshifumi; Balakrish Nair, Gopinath; Arimoto, Sakae; Negishi, Tomoe; Okamoto, Keinosuke

<P><B>ABSTRACT&ensp;</B></P><P><I>Aeromonas</I> have been isolated from a wide variety of aquatic environments. However the number of <I>Aeromonas</I> in sea water is extremely small compared to that in fresh water. In <I>in vitro</I> culture, <I>Aeromonas</I> can grow in mediums containing NaCl at a concentration of 3.0%, this concentration corresponding to that of sea water. It is unclear why the number of <I>Aeromonas</I> is low in sea water. Exoproteins of bacteria are thought to be important for bacterial growth and survival in the environment. Previously, the present authors have shown that mediums containing 3.0% NaCl suppress production of two proteases, serine protease and metalloprotease. In this experiment, other exoproteins whose production is influenced by the amount of NaCl in the medium were analyzed. A protein whose production is repressed in medium containing 3.0% NaCl was found and purified. Biological assay of the purified protein showed that it degrades tributyrin and hydrolyzes <I>para&#8208;</I>nitrophenyl&#8208;fatty acylesters. These results show that the protein is a lipase. Subsequently, the nucleotide sequence of the gene encoding the lipase was determined and the amount of mRNA of the lipase gene in the cells measured. It was found that transcription of the gene is not inhibited by NaCl in the medium. This result indicates that the lipase might be synthesized, but the folding process to become an active structure does not progress smoothly in a medium containing 3.0% NaCl.</P>

2012

Blackwell Publishing Asia

0385-5600

1348-0421

56

5

pp.295-307

10.1111/j.1348-0421.2012.00445.x

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63150875

Aeromonas sobria; phospholipase A1; sodium chloride