초록
<P><B>Abstract</B></P><P>We report a simple, mild, and synthetically clean approach to accelerate the rate of enzymatic oxidation reactions by a factor of up to 100 when compared to conventional batch gas/liquid systems. Biocatalytic decomposition of H<SUB>2</SUB>O<SUB>2</SUB> is used to produce a soluble source of O<SUB>2</SUB> directly in reaction media, thereby enabling the concentration of aqueous O<SUB>2</SUB> to be increased beyond equilibrium solubility under safe and practical conditions. To best exploit this method, a novel flow reactor was developed to maximize productivity (g product L<SUP>−1</SUP> h<SUP>−1</SUP>). This scalable benchtop method provides a distinct advantage over conventional bio‐oxidation in that no pressurized gas or specialist equipment is employed. The method is general across different oxidase enzymes and compatible with a variety of functional groups. These results culminate in record space‐time yields for bio‐oxidation.</P>