BioChem - DOS

Biosynthesis of uniformly labeled 13C- and 14C-arachidonic acid in Mortierella alpina

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Lee, J.V.; Furman, R.; Axelsen, P.H.

Arachidonic acid (ARA) is one of the most abundant polyunsaturated fatty acids (PUFAs) in the mammalian brain. Many enzymatically- and nonenzymatically-produced metabolic products have important and potent pharmacological properties. However, uniformly isotope labeled forms of ARA are not commercially available for studying the metabolic fates of ARA. This study describes a simple and efficient protocol for the biosynthesis of U-<SUP>13</SUP>C-ARA from U-<SUP>13</SUP>C-glucose, and U-<SUP>14</SUP>C-ARA from U-<SUP>14</SUP>C-glucose by Mortierella alpina. The protocols yield approximately 100nmol quantities of U-<SUP>13</SUP>C-ARA with an isotopic purity of 95% from a 500&mu;l batch volume, and approximately 2&mu;Ci quantities of U-<SUP>14</SUP>C-ARA with an apparent specific activity in excess of 1200Ci/mol from a 250&mu;l batch volume.

2017

Elsevier Applied Science

0960-8524

227

pp.142-146

10.1016/j.biortech.2016.12.050

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77178975

https://europepmc.org/articles/pmc5237611?pdf=render

Mortierella alpina; Arachidonic acid; Carbon isotope labeling; Micro-scale culture