초록
Photobiological H<SUB>2</SUB> production is a promising method for renewable energy development. An innovative system that co-cultivating Rhodopseudomonas palustris WP3-5 and Anabaena sp. CH3 was carried out to estimate the effect of co-cultivation on H<SUB>2</SUB> production enhancement. H<SUB>2</SUB> production prolongation and enhancement were observed due to the light and metabolic compatibility of these two strains. Co-culture system served by acetate and fructose as carbon source can accumulate H<SUB>2</SUB> in 140.8 mL, almost double than the sum of individuals. Moreover, the enhancement of H<SUB>2</SUB> production was significantly affected by the mixed ratio of two strains. The mixed ratio (WP3-5:CH3) of 1:2 showed a highest H<SUB>2</SUB> production rate in 44.8 mL-H<SUB>2</SUB>/L-culture/h, and both 2:1 and 1:2 exhibited a relatively high substrate conversion efficiency during the latest period of cultivation, whereas the mixed ratio of 1:1 and 3:1 only revealed a prolongation in H<SUB>2</SUB> production due to metabolic compatibility of two strains.