Improved xylitol production by expressing a novel D-arabitol dehydrogenase from isolated Gluconobacter sp. JX-05 and co-biotransformation of whole cells
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Improved xylitol production by expressing a novel D-arabitol dehydrogenase from isolated Gluconobacter sp. JX-05 and co-biotransformation of whole cells
Qi, X.; Zhang, H.; Magocha, T.A.; An, Y.; Yun, J.; Yang, M.; Xue, Y.; Liang, S.; Sun, W.; Cao, Z.
초록
In the present study, a novel ardh gene encoding d-arabitol dehydrogenase (ArDH) was cloned and expressed in Escherichia coli from a new isolated strain of Gluconobacter sp. JX-05. Sequence analysis revealed that ArDH containing a NAD(P)-binding motif and a classical active site motif belongs to the short-chain dehydrogenase family. Subsequently, the optimal pH and temperature, specific activities and kinetic parameter of ArDH were determined. In the co-biotransformation by the whole cells of BL21-ardh and BL21-xdh, 26.1g/L xylitol was produced from 30g/L d-arabitol in 22h with a yield of 0.87g/g. The xylitol production was increased by more than two times as compared with that of Gluconobacter sp. alone, and was improved 10.1% than that of Gluconobacter sp. mixed with BL21-xdh.