초록
<P>Three newly discovered H<SUB>2</SUB> producing bacteria namely <I>Clostridium perfringens</I> strain JJC, <I>Clostridium bifermentans</I> strain WYM and <I>Clostridium</I> sp. strain Ade.TY originated from landfill leachate sludge have demonstrated highly efficient H<SUB>2</SUB> production. The maximum H<SUB>2</SUB> production attained from these isolates are in the descending order of strain <I>C. perfringens</I> strain JJC > <I>C. bifermentans</I> strain WYM > <I>Clostridium</I> sp. strain Ade.TY with yield of 4.68 ± 0.12, 3.29 ± 0.11, and 2.87 ± 0.10 mol H<SUB>2</SUB>/mol glucose, respectively. The result has broken the conventional theoretical yield of 4 mol H<SUB>2</SUB>/mol glucose. These isolates were thermodynamically favourable with Gibbs free energy between −33 and −35 kJ/mol (under process conditions: pH 6, 37 °C and 5 g/L glucose). All three isolates favour butyrate pathway for H<SUB>2</SUB> production with the ratio of acetate and butyrate of 0.77, 0.65 and 0.80 for strain JJC, WYM and Ade.TY, respectively. This study reported provides a new insight on the potential of unique bacteria in H<SUB>2</SUB> production.</P>