초록
Spent coffee grounds (SCG) represent the main coffee industry residues with a great potential to be reutilized in various biotechnological processes. In this study, several carotenogenic yeasts strains were exploited for the production of vitamin-enriched biomass, cultivating in SCG-based media. The fermentation was firstly carried out in Erlenmeyer flasks in order to select the best biomass and pigment producer. Among four tested strains, Sporobolomyces roseus showed the highest potential for the accumulation of carotenoids. Maximum pigment concentration and yield was obtained when cultivating in SCG-based media, 12.59mgl<SUP>-1</SUP> and 1.26mgg<SUP>-1</SUP>, respectively. Comparing both, the batch and the fed-batch cultivation modes, the strategy of sequential addition of pre-concentrated SCG media in the bioreactor gave higher biomass yield (maximum 41gl<SUP>-1</SUP> during 41-48h after the beginning of fermentation). Thus, SCG can be considered as potentially promising industrial waste stream for economically feasible production of enriched yeasts biomass.