BioChem - DOS

A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of β-carotene

Biotechnology and bioengineering

Larroude, Macarena; Celinska, Ewelina; Back, Alexandre; Thomas, Stephan; Nicaud, Jean‐ Marc; Ledesma‐ Amaro, Rodrigo

<P><B>Abstract</B></P><P>The increasing market demands of &beta;&#8208;carotene as colorant, antioxidant and vitamin precursor, requires novel biotechnological production platforms. <I>Yarrowia lipolytica</I>, is an industrial organism unable to naturally synthesize carotenoids but with the ability to produce high amounts of the precursor Acetyl&#8208;CoA. We first found that a lipid overproducer strain was capable of producing more &beta;&#8208;carotene than a wild type after expressing the heterologous pathway. Thereafter, we developed a combinatorial synthetic biology approach base on Golden Gate DNA assembly to screen the optimum promoter&#8208;gene pairs for each transcriptional unit expressed. The best strain reached a production titer of 1.5 g/L and a maximum yield of 0.048 g/g of glucose in flask. &beta;&#8208;carotene production was further increased in controlled conditions using a fed&#8208;batch fermentation. A total production of &beta;&#8208;carotene of 6.5 g/L and 90 mg/g DCW with a concomitant production of 42.6 g/L of lipids was achieved. Such high titers suggest that engineered <I>Y. lipolytica</I> is a competitive producer organism of &beta;&#8208;carotene.</P>

2018

0006-3592

1097-0290

115

2

pp.464-472

10.1002/bit.26473

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART79345135

β&#x2010; carotene; golden gate; metabolic engineering; promoter shuffling; synthetic biology; Yarrowia lipolytica;