BioChem - DOS

Cultivation of Pichia capsulata as a whole-cell biocatalyst with NADH-dependent alcohol dehydrogenase activity for R-1-phenylethanol production

Food and bioproducts processing : transactions of the Institution of Chemical Engineers, Part C

Homola, P.; Kurak, T.; Illeova, V.; Polakovic, M.

The aim of this work was to develop a cultivation process for the production of NADH-dependent alcohol dehydrogenase catalyzing a less common reduction of acetophenone to R-1-phenylethanol, an important compound for fragrance and flavor industry. Screening of 23 yeast strains showed that Pichia capsulata ATCC 16753 cells possess high catalytic activity for acetophenone reduction to R-1-phenylethanol. This high alcohol dehydrogenase activity was observed not only for whole cells but also for a raw enzyme solution using NADH as the cofactor. R-1-phenylethanol was produced in enantiomeric excess values of over 99% for the raw enzyme solution and of over 90% for the whole-cell biocatalyst cultivated at optimal conditions. The key factors for achieving high yield and selectivity were: proper cultivation time, glucose concentration and dissolved oxygen control.

2015

The Institution ; Hemisphere Pub. Corp. [distributor]

0960-3085

96

pp.126-132

10.1016/j.fbp.2015.07.007

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART73324121

Alcohol dehydrogenase production; Bioreactor cultivation; Chiral reduction; Pichia capsulata; R-1-phenylethanol; Whole-cell biocatalysis