초록
An isolated Klebsiella sp. HE1 strain was used to produce H<SUB>2</SUB> and other energy-rich soluble metabolites from glycerol at different pH values, temperatures, and glycerol concentrations. The highest cumulative hydrogen production (6.89l), H<SUB>2</SUB> production rate (11.76mmol/h/l), and hydrogen yield (0.345molH<SUB>2</SUB>/mol glycerol) were obtained at 35<SUP>o</SUP>C, pH 6.0, and an initial glycerol concentration (C<SUB>gly</SUB>) of 50g/l. These conditions are also preferable for producing ethanol, 1,3-propanediol, and 2,3-butanediol with the highest production rate of 6.80 (C<SUB>gly</SUB>=50g/l)mmol/h/l, 6.95 (C<SUB>gly</SUB>=70g/l)mmol/h/l and 1.17 (C<SUB>gly</SUB>=50g/l)mmol/h/l, respectively, and the highest yields of 0.8 (C<SUB>gly</SUB>=10g/l), 0.37 (C<SUB>gly</SUB>=70g/l), and 0.08 (C<SUB>gly</SUB>=70g/l) mol/mol glycerol, respectively. Combining the gaseous (H<SUB>2</SUB>) and liquid fuels (alcohols), the highest total energy generation rate was 26.7kJ/h/l obtained at C<SUB>gly</SUB>=70g/l, while the best total energy yield was 0.90kJ/kJ at C<SUB>gly</SUB>=50g/l.