BioChem - DOS

Characterization of a thermostable xylanase from a newly isolated Kluyvera species and its application for biobutanol production

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Xin, F.; He, J.

Kluyvera species strain OM3 isolated from spent mushroom substrate could produce a high level of cellulase-free xylanase (5.12U/mL). This xylanase showed maximum activities at 70<SUP>o</SUP>C and pH 8.0, which could retain 100% and 71% activity after 1h incubation at 60<SUP>o</SUP>C and 70<SUP>o</SUP>C, and maintain stability over a wide range of pHs (5.0-9.0), indicating its thermal and pH stability. Moreover, the xylanase could hydrolyze untreated lignocellulosics (e.g., palm oil fiber) to reducing sugars with a yield of 27.1-46.9mg/g. A co-culture consisting of Kluyvera sp. strain OM3 and Clostridium sp. strain BOH3 could directly convert birchwood xylan to 1.2g/L butanol, which was comparable to the amount of butanol (1.7g/L) generated via separate hydrolysis by the xylanase and fermentation by Clostridium sp. strain BOH3. This is the first report on the production, characterization of a xylanase from genus Kluyvera and its application for butanol production directly from hemicelluloses.

2013

Elsevier Applied Science

0960-8524

135

pp.309-315

10.1016/j.biortech.2012.10.002

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65836468

Xylanase; Kluyvera; Clostridium; Hemicelluloses; Butanol