초록
<P><B>Abstract</B></P> <P>Fermentation media optimisation for 2,3-butanediol (BDO) production from sugarcane molasses with <I>Enterobacter ludwigii</I> has been conducted. Plackett-Burman design, central composite design and constrained optimisation were combined to optimise nutrient composition leading to enhanced BDO production efficiency. (NH<SUB>4</SUB>)<SUB>2</SUB>SO<SUB>4</SUB>, yeast extract and KOH were the most significant media components affecting productivity. Constrained optimisation was applied to maximise productivity and maintain BDO concentration, yield and BDO to by-products ratio at desirable levels. Fed-batch fermentations carried out with sugarcane molasses at optimum pH (6.3) and temperature (33.9 °C) with the optimised media led to the production of BDO concentration, yield and productivity of 50.6 g/L, 0.31 g/g and 2.66 g L<SUP>−1</SUP> h<SUP>-1</SUP>, respectively. The minimum selling price of BDO at 10,000 t and 50,000 t annual production capacity is $2.66 - $3.82/kg and $2.25 - $3.23/kg, respectively, when sugarcane molasses is used at a market price of $0.05 - $0.2/kg.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fermentation medium optimisation for BDO production using sugarcane molasses. </LI> <LI> Experimental design was employed to optimise medium composition. </LI> <LI> BDO concentration reached 50.6 g/L with productivity of 2.66 g L<SUP>−1</SUP> h<SUP>−1</SUP>. </LI> <LI> The BDO minimum selling price is $3.82/kg at 10,000 t annual production capacity. </LI> </UL> </P>