초록
<P><B>Highlights</B></P><P>► We cloned a 2,3-butanediol dehydrogenase gene and expressed it in <I>Escherichia coli</I>. ► We confirmed the stereospecificity of the 2,3-butanediol dehydrogenase. ► We used the engineered <I>E. coli</I> to produce (2<I>S</I>,3<I>S</I>)-2,3-butanediol from diacetyl. ► The concentration of (2<I>S</I>,3<I>S</I>)-2,3-butanediol produced was higher than other reports.</P> <P><B>Abstract</B></P><P>(2<I>S</I>,3<I>S</I>)-2,3-Butanediol ((2<I>S</I>,3<I>S</I>)-2,3-BD) is a crucial chiral compound that acts as an excellent building block in asymmetric synthesis of highly valuable chiral compounds. However, the low concentration and optical purity of (2<I>S</I>,3<I>S</I>)-2,3-BD produced in previous studies limited its applications. In the present work, the gene encoding 2,3-butanediol dehydrogenase from an <I>Enterobacter cloacae</I> ssp<I>. dissolvens</I> strain SDM was cloned and expressed in <I>Escherichia coli</I>. Whole cells of the recombinant <I>E. coli</I> was used to produce (2<I>S</I>,3<I>S</I>)-2,3-BD from diacetyl. Under optimal conditions, high-optical-purity (2<I>S</I>,3<I>S</I>)-2,3-BD (purity >99%) was obtained with concentrations of 16.1gl<SUP>−1</SUP> and 26.8gl<SUP>−1</SUP> in batch and fed-batch conversions, respectively. Thus, the process might be a promising alternative for the production of (2<I>S</I>,3<I>S</I>)-2,3-BD.</P>