BioChem - DOS

Dark fermentative hydrogen production from xylose by a hot spring enrichment culture

International journal of hydrogen energy

Makinen, A.E.; Nissila, M.E.; Puhakka, J.A.

Dark fermentative hydrogen production by a hot spring culture was studied from different sugars in batch assays and from xylose in continuous stirred tank reactor (CSTR) with on-line pH control. Batch assays yielded hydrogen in following order: xylose > arabinose > ribose > glucose. The highest hydrogen yield in batch assays was 0.71 mol H<SUB>2</SUB>/mol xylose. In CSTR the highest H<SUB>2</SUB> yield and production rate at 45 <SUP>o</SUP>C were 1.97 mol H<SUB>2</SUB>/mol xylose and 7.3 mmol H<SUB>2</SUB>/h/L, respectively, and at 37 <SUP>o</SUP>C, 1.18 mol H<SUB>2</SUB>/mol xylose and 1.7 mmol H<SUB>2</SUB>/h/L, respectively. At 45 <SUP>o</SUP>C, microbial community consisted of only two bacterial strains affiliated to Clostridium acetobutulyticum and Citrobacter freundii, whereas at 37 <SUP>o</SUP>C six Clostridial species were detected. In summary hydrogen yield by hot spring culture was higher with pentoses than hexoses. The highest H<SUB>2</SUB> production rate and yield and thus, the most efficient hydrogen producing bacteria were obtained at suboptimal temperature of 45 <SUP>o</SUP>C for both mesophiles and thermophiles.

2012

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

37

17

pp.12234-12240

10.1016/j.ijhydene.2012.05.158

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63783046

Biohydrogen; Xylose; Clostridium acetobutylicum; Citrobacter freundii; Dark fermentation