초록
Cellulase immobilized on silica through the assistance of l-cysteine functionalized gold nano-particle was applied for the continuous hydrolysis of waste bamboo chopsticks powder to produce glucose. The optimal conditions for the continuous hydrolysis were pH 8.0, 50<SUP>o</SUP>C. A 4-day reaction with an initial 0.3gL<SUP>-1</SUP> waste bamboo chopsticks powder, a feed containing 0.2gL<SUP>-1</SUP> waste bamboo chopsticks powder at a continuous feed and draw rate of 0.5mLmin<SUP>-1</SUP>, and an enzyme loading of 40mgcellulase(gsilica)<SUP>-1</SUP>, has 72.0-76.6% conversion rates of repeated hydrolyses that correspond to a total production of 630.5-671.2mg glucose and are much better than batch hydrolyses. At higher enzyme loading (117mgcellulase(gsilica)<SUP>-1</SUP>), higher initial concentration (0.5gL<SUP>-1</SUP>), and higher feed concentration (0.42gL<SUP>-1</SUP>) the conversion rate increases to 82.9% and a total amount of 1418mgglucose. The immobilized cellulase can be recovered easily by filtration and used repeatedly at least 6 times over a period more than 90 days with a recovered activity approximately the same as or better than previous reactions. Thus the process is promising for scaling up.