초록
<P><B>Abstract</B></P> <P>In this study, a newly strain named <I>Clostridium butyricum</I> YJH-09 were isolated from the sample of pond soil and identified through physiological, biochemical and 16S rDNA analysis. Then, the <I>dhaT</I> gene encoding a novel 1,3-propanediol dehydrogenase (PDOR) was cloned from this strain and expressed in <I>Escherichia coli</I> BL21(DE3). Subsequently, the recombinant PDOR was purified and the optimal pH and temperature, specific activities and kinetic parameter were investigated. Furthermore, the whole cells of <I>Clostridium butyricum</I> YJH-09 mixed with BL21-<I>dhaT</I> were used to produce 1,3-PD through co-biotransformation. As results, 25.88g/L of 1,3-PD was generated with 0.54g/g yield from 50g/L glycerol in 30h, and the 1,3-PD production was increased more than 2-fold compared with wild type strain alone. This research would offer useful information for further development of the biosynthesis of 1,3-PD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A newly <I>dhaT</I> gene from isolated <I>C. butyricum</I> YJH-09 was cloned and expressed. </LI> <LI> The mixed whole cells was firstly used for producing 1,3-PD based on a novel characterized PDOR. </LI> <LI> The production of 1,3-PD was improved more than 2 times via co-biotransformation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>