초록
<P><B>Abstract</B></P><P>To enhance biohydrogen production, <I>Clostridium beijerinckii</I> was co‐cultured with <I>Geobacter metallireducens</I> in the presence of the reduced extracellular electron shuttle anthrahydroquinone‐2, 6‐disulfonate (AH<SUB>2</SUB>QDS). In the co‐culture system, increases of up to 52.3% for maximum cumulative hydrogen production, 38.4% for specific hydrogen production rate, 15.4% for substrate utilization rate, 39.0% for substrate utilization extent, and 34.8% for hydrogen molar yield in co‐culture fermentation were observed compared to a pure culture of <I>C. beijerinckii</I> without AH<SUB>2</SUB>QDS. <I>G. metallireducens</I> grew in the co‐culture system, resulting in a decrease in acetate concentration under co‐culture conditions and a presumed regeneration of AH<SUB>2</SUB>QDS from AQDS. These co‐culture results demonstrate metabolic crosstalk between the fermentative bacterium <I>C. beijerinckii</I> and the respiratory bacterium <I>G. metallireducens</I> and suggest a strategy for industrial biohydrogen production. Biotechnol. Bioeng. 2013; 110: 164–172. © 2012 Wiley Periodicals, Inc.</P>