BioChem - DOS

Efficient fructose production from plant extracts by immobilized inulinases from Kluyveromyces marxianus and Helianthus tuberosus

International journal of biological macromolecules

Holyavka, M.G.; Kayumov, A.R.; Baydamshina, D.R.; Koroleva, V.A.; Trizna, E.Yu.; Trushin, M.V.; Artyukhov, V.G.

<P><B>Abstract</B></P> <P>The enzymatic hydrolysis of poly- and oligosaccharides from plants seems like an advantageous approach for sugars production. Two inulinases producing fructose from plant oligosaccharides were isolated from yeast <I>Kluyveromyces marxianus</I> and plant <I>Helianthus tuberosus</I>. Both enzymes were immobilized on polymeric carriers by using the static adsorption approach. We could save 80.4% of the initial catalytic activity of plant inulinase immobilized on KU-2 cation-exchange resin and 75.5% of yeast enzyme activity adsorbed on AV-17-2P anion-exchange resin. After immobilization, the Km values increased 1.5 and 6 times for enzymes from <I>K. marxianus</I> and <I>H. tuberosus</I>, respectively. The optimal temperatures for catalysis of both enzymes were increased from 48&ndash;50 &deg;C up to 70 &deg;C. The activities of both immobilized enzymes remained unchanged after the 10 cycles of 20-min hydrolysis reaction at 70 &deg;C model batch reactor. Sorbents, native and immobilized enzymes did not exhibit any mutagenic or cytotoxic activity.</P>

2018

Elsevier

0141-8130

1879-0003

115

pp.829-834

10.1016/j.ijbiomac.2018.04.107

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART85082406

Inulinase; Immobilization; Fructose