초록
<P><B>Summary</B></P><P><I>Saccharomyces cerevisiae</I> cannot produce extracellular lipase and utilize low‐cost lipid substrates. This study aimed to express extracellular lipase from <I>Yarrowia lipolytica</I> in <I>S. cerevisiae</I>, construct recombinant oily substrate consumer strains, and compare the roles of native and mutant <I>Y. lipolytica</I> extracellular lipases in <I>S. cerevisiae</I>. The <I>LIP2</I> gene of <I>Y. lipolytica</I> DSM3286 and its mutant <I>Y. lipolytica</I> U6 were isolated and cloned by expression vector in <I>S. cerevisiae</I>. New recombinant <I>S. cerevisiae</I> strains FDS100 containing the native <I>LIP2</I> gene, and FDS101 containing the mutant <I>LIP2</I> gene were produced 10 and 15 U ml <SUP>−1</SUP> extracellular lipase respectively, on a production medium containing olive oil. New recombinant <I>S. cerevisiae</I> strains produce acceptable amount of extracellular lipase in comparison with <I>Y. lipolytica</I> wild‐type strains. These strains can utilize olive oil and lipids as low‐cost substrates to produce bioethanol, single cell protein and other biotechnologically valuable products. The recombinant <I>S. cerevisiae</I> strain with mutant <I>LIP2</I> produced lipase with 1.5‐fold higher activity. The <I>LIP2</I> gene of <I>Y. lipolytica</I> was expressed in <I>S. cerevisiae</I> as a heterologous protein without any modifications. Strong components of the <I>Y. lipolytica</I> expression/secretion system could be used for high‐level production of recombinant proteins in <I>S. cerevisiae</I>.</P>