초록
<P><B>Abstract</B></P> <P>The diploid <I>Miscanthus sinensis</I> “Shiozuka” which was selected as a high-biomass producing line, and the triploid <I>M.</I> × <I>giganteus</I> (M×G) were treated by ball milling (physical treatment) and alkaline hydrogen peroxide treatment (AHP; chemical treatment), and their structural sugar compositions and enzymatic digestibility were compared. The structural sugar content of Shiozuka was moderate and lower than that of M×G. The Klason lignin content of Shiozuka was also lower than that of M×G. However, Shiozuka was sensitive to ball milling and AHP treatment; ball milled and AHP-treated Shiozuka had higher enzymatic digestibility than ball milled and AHP-treated M×G. Shiozuka would be promising feedstock to obtain fermentable sugars with low energy consumption. Finally, enzymes for the hydrolysis of chemically treated <I>Miscanthus</I> were isolated from <I>Trichoderma reesei</I> ATCC 66589 and <I>Penicillium pinophilum</I>. The sugar yield could be increased by enzymatic hydrolysis of AHP-treated samples with NaOH and H<SUB>2</SUB>O<SUB>2</SUB> and the isolated enzymes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Miscanthus sinensis</I> “Shiozuka” contained moderate sugar but low lignin contents. </LI> <LI> Shiozuka was more sensitive to physical and chemical treatments than M×G. </LI> <LI> Enzymatic digestibilities of the pretreated Shiozuka were higher than those of M×G. </LI> <LI> Shiozuka is a promising bioenergy grass cultivar. </LI> </UL> </P>