BioChem - DOS

Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp. by the Expression of Vitreoscilla Hemoglobin

Journal of agricultural and food chemistry

Suen, Yung Lee; Tang, Hongmei; Huang, Junchao; Chen, Feng

<P>Dissolved oxygen is a critical factor for heterotrophic cell growth and metabolite production. The aim of this study was to investigate the effects of an oxygen-involved protein on cell growth and fatty acid and astaxanthin production in the biologically important thraustochytrid <I>Aurantiochytrium</I> sp. The hemoglobin of the <I>Vitreoscilla stercoraria</I> (VHb) gene was fused upstream with a zeocin resistance gene (<I>ble</I>) and driven by the <I>Aurantiochytrium</I> tubulin promoter. The expression construct was introduced into two strains of <I>Aurantiochytrium</I> sp. by electroporation. Transgenic <I>Aurantiochytrium</I> sp. strains MP4 and SK4 expressing the heterologous VHb achieved significantly higher maximum biomass than their corresponding controls in microaerobic conditions. Furthermore, the transformants of <I>Aurantiochytrium</I> sp. SK4 produced 44% higher total fatty acid and 9-fold higher astaxanthin contents than the wild type control in aerobic conditions. The present study highlights the biotechnological application of VHb in high-cell density fermentation for enhanced biomass production as well as high-value metabolites.</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2014/jafcau.2014.62.issue-51/jf5048578/production/images/medium/jf-2014-048578_0005.gif'></P>

2014

American Chemical Society

0021-8561

1520-5118

62

51

pp.12392-12398

10.1021/jf5048578

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71127653

astaxanthin; Aurantiochytrium; fatty acid; Vitreoscilla hemoglobin; thraustochytrids