초록
<P><B>Abstract</B></P> <P>The autochthonous bacterial strain was isolated from banana waste (BW) for hydrogen production and organic acids from different pure substrates and BW. The potential production of H<SUB>2</SUB> and metabolic pathways were analyzed at pH 7.0 at 37 °C. Facultative anaerobic bacterium similar to <I>Bacillus</I> sp. was identified<U>,</U> with generation time (Tg) and growth rate (μ) of 0.43 h and 1.60 h<SUP>−1</SUP>, respectively, from glucose. The hydrogen production (<I>P</I>) using pure substrates was observed between 10.81 mmol.L<SUP>−1</SUP> and 17.75 mmol.L<SUP>−1</SUP> from xylose and maltose, respectively. The biggest and smallest hydrogen <I>P</I> of 18.77 mmol.L<SUP>−1</SUP> and 1.72 mmol.L<SUP>−1</SUP>, were obtained with 3.5 and 0.5 g.L<SUP>−1</SUP> of cellobiose, respectively. The highest hydrogen yield (126.93 mL.g<SUP>−1</SUP> <SUB>carbohydrates added</SUB>) was obtained with 2 g.L<SUP>−1</SUP> cellobiose. In the assay using banana waste (5 g.L<SUP>−1</SUP>) the maximum <I>P</I> and yield (YH<SUB>2</SUB>) of 31.7 mmol.L<SUP>−1</SUP> and 94.66 mL H<SUB>2</SUB> g<SUP>−1</SUP> <SUB>carbohydrates added</SUB>, was obtained respectively. The main metabolic pathway of hydrogen production by <I>Bacillus</I> sp. RM1 from banana waste was acetic-butyric acid of 487.69 g.L<SUP>−1</SUP> and 535.88 g.L<SUP>−1</SUP>, respectively. The accessibility of various carbon sources by <I>Bacillus</I> sp. RM1 in fermentation can benefit the hydrogen production from complex organic substrates in the bioaugmentation process.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bacillus sp. RM1 was isolated from banana waste (BW). </LI> <LI> H2 yield of 126.93 mL H2 g-1carbohydrates added was achieved for 2.5 g cellobiose.L-1. </LI> <LI> The H2 yield from BW was 1.8 times higher than using pure sugars. </LI> <LI> End products from BW conversion were acetic acid, butyric acid, caproic acid and ethanol. </LI> <LI> Maximum H2 production of 31.7 mmol.L-1 was obtained from fresh BW in 20 h. </LI> </UL> </P>