초록
<P><B>Highlights</B></P><P>► Expanded-bed adsorption was integrated with in the fermentation process viable for <I>in situ</I> extracellular product removal. ► The coupled fermentation–separation system could overcome product inhibition. ► The final titer of 145.2gl<SUP>−1</SUP> succinic acid was obtained after 126h fermentation.</P> <P><B>Abstract</B></P><P>An integrated fermentation process for the production of succinic acid by <I>Actinobacillus succinogenes</I> at high titer, yield and productivity was developed by applying <I>in situ</I> product removal (ISPR) strategy. The ISPR process was conducted by a coupled expanded-bed adsorption (EBA) system. The novel approach during the product inhibitory period of microbial fermentation enhanced the cell growth of <I>A. succinogenes</I> from 48h to 126h, and significantly increased succinic acid production up to the final titer of 145.2gl<SUP>−1</SUP> with an average yield of 0.52gg<SUP>−1</SUP>, and productivity of 1.3gl<SUP>−1</SUP>h<SUP>−1</SUP>. The maximum yield and productivity reached 0.76gg<SUP>−1</SUP> and 2.58gl<SUP>−1</SUP>h<SUP>−1</SUP>, respectively after the first ISPR operation cycle. ISPR process with EBA leads to the production of succinct acid via fermentation economically feasible.</P>