초록
<P><B>Abstract</B></P><P>Feasibility of producing (<I>R</I>)-3-hydroxybutyric acid ((<I>R</I>)-3-HB) using wild type <I>Azohydromonas lata</I> and its mutants (derived by UV mutation) was investigated. <I>A. lata</I> mutant (M5) produced 780mg/l in the culture broth when sucrose was used as the carbon source. M5 was further studied in terms of its specificity with various bioconversion substrates for production of (<I>R</I>)-3-HB. (<I>R</I>)-3-HB concentration produced in the culture broth by M5 mutant was 2.7-fold higher than that of the wild type strain when sucrose (3% w/v) and (<I>R,S</I>)-1,3-butanediol (3% v/v) were used as carbon source and bioconversion substrate, respectively. Bioconversion of resting cells (M5) with glucose (1% v/w), ethylacetoacetate (2% v/v), and (<I>R,S</I>)-1,3-butanediol (3% v/v), resulted in (<I>R</I>)-3-HB concentrations of 6.5g/l, 7.3g/l and 8.7g/l, respectively.</P>