Use of Low-Cost Agro-Industrial Substrate to Obtain Carotenoids from Phaffia rhodozyma in a Bioreactor

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- 화장품용 기능성소재
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  2. 계면활성제⁄증점제
- 의료용 화학소재
  1. 건강보조식품

논문

Use of Low-Cost Agro-Industrial Substrate to Obtain Carotenoids from Phaffia rhodozyma in a Bioreactor

학술지

Industrial biotechnology

저자명

Urnau, Letí cia; Colet, Rosicler; Reato, Paloma Truccolo; Fernandes de Medeiros Burkert, Janaí na; Rodrigues, Eliseu; Gomes, Raul; Jacques, Rosâ ngela Assis; Valduga, Eunice; Steffens, Clarice

초록

AbstractThe aim of this work was to evaluate the production of carotenoids from red yeast Phaffia rhodozyma in a bioreactor using agro-industrial wastes. This study used a sequential strategy of experimental design (Placket-Burman design and central composite rotatable design) to maximize carotenoids bioproduction using agro-industrial byproducts and to optimize agitation and aeration rates in bioreactor. The kinetic and stoichiometric parameters of bioproduction were studied, and the carotenoid profile was assessed by high-performance liquid chromatography coupled with a mass spectrometry detector. The maximum total carotenoid recuperation was 2,380 μg/L (639 μg/g on dry basis) reached after 60 h. Four carotenoids were identified, with β-carotene (95%) being the major carotenoid produced using study conditions (250 rpm agitation, 1.5 vvm aeration, 96 h, 25°C) and substrate (100 g/L glycerol, 100 g/L corn steep liquor, and 20 g/L rice parboiled water).

발행연도

2019

발행기관

Mary Ann Liebert, Inc., publishers

ISSN

1550-9087

권

호

페이지

pp.25-34

DOI

10.1089/ind.2018.0027