Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics
학술지
International journal of biological macromolecules
<P><B>Abstract</B></P> <P>In this study, two xylanase genes (GH10 and GH11) derived from <I>Malbranchea cinnamomea</I>, designated as XYN10A_MALCI and XYN11A_MALCI, respectively, were expressed in <I>Pichia pastoris</I> X33. The maximum level of xylanase expression was found to be 24.3U/ml for <B>r</B>XYN10A_MALCI and 573.32U/ml for <B>r</B>XYN11A_MALCI. The purified recombinant <B>r</B>XYN11A_MALCI was stable at 70°C and catalytically active against a variety of substituted (arabinoxylans) as well as unsubstituted xylans. The hydrolytic potential of recombinant xylanases for enhancing the hydrolysis of acid/alkali pretreated lignocellulosics (rice straw and bagasse) by the commercial cellulase Cellic CTec2 was assessed which revealed that both <B>r</B>XYN10A_MALCI and <B>r</B>XYN11A_MALCI act synergistically with commercial cellulases and resulted in 1.54 and 1.58 folds improved hydrolysis of acid treated rice straw and alkali treated rice straw using cocktail comprising of Cellic CTec2 and XYN11A_MALCI (8:2 ratio) when compared to Cellic CTec2 alone at same protein loading rate of (∼5.7mg/g biomass).</P> <P><B>Highlights</B></P> <P> <UL> <LI> Xylanases (GH10 and GH11) from <I>M. cinnamomea</I> cloned and expressed in <I>P. pastoris</I>. </LI> <LI> Xylanase GH11 highly thermostable and catalytically efficient. </LI> <LI> Cocktails of GH10/GH11 with Cellic CTec2 showed enhanced hydrolysis of cellulosics. </LI> </UL> </P>