BioChem - DOS

Chrysoporthe cubensis: A new source of cellulases and hemicellulases to application in biomass saccharification processes

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Falkoski, D.L.; Guimaraes, V.M.; de Almeida, M.N.; Alfenas, A.C.; Colodette, J.L.; de Rezende, S.T.

The plant pathogenic fungus Chrysoporthe cubensis was cultivated under solid state employing different substrates and the highest endoglucanase (33.84Ug<SUP>-1</SUP>), FPase (2.52Ug<SUP>-1</SUP>), &beta;-glucosidase (21.55Ug<SUP>-1</SUP>) and xylanase (362.38Ug<SUP>-1</SUP>) activities were obtained using wheat bran as carbon source. Cellulases and xylanase produced by C. cubensis showed maximal hydrolysis rate at pH 4.0 and in a temperature range of 50-60<SUP>o</SUP>C. All enzymatic activities were highly stable at 40 and 50<SUP>o</SUP>C through 48h of pre-incubation. Saccharification of alkaline pretreated sugarcane bagasse by crude enzyme extract from C. cubensis resulted in release of 320.8mg/g and 288.7mg/g of glucose and xylose, respectively. On another hand, a similar assay employing commercial cellulase preparation resulted in release of 250.6mg/g and 62.1mg/g of glucose and xylose, respectively. Cellulolytic extract from C. cubensis showed a great potential to be used in biomass saccharification processes.

2013

Elsevier Applied Science

0960-8524

130

pp.296-305

10.1016/j.biortech.2012.11.140

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65520646

Chrysoporthe cubensis; Plant pathogen; Cellulases; Hemicellulases; Biomass saccharification