초록
<P>The development and utilization of energy has played a key role in the human civilization process, and fossil fuels have become the major energy supply after Industrial Revolution. However, with the massive utilization of fossil fuels, petroleum and natural gas will be exhausted, and people will face the risk of energy shortages. For this reason, the development of clean, efficient, and renewable energy should be a priority. Hydrogen is considered a safe and secure energy source for human health and the global environment, as it does not emit any pollutants while burning. Hence, this study combines biohydrogen production of dark fermentation with ultrasonic to explore the effects of ultrasonic frequency, amplitude and exposure time on biohydrogen production of dark fermentation. The experimental results show that the largest biohydrogen production appears when the ultrasonic frequency being 1MHz and the ultrasonic exposure time is 15 min for exposure and 15 min for termination. It is therefore possible to find the optimal hydrogen production condition and to further understand the effect of ultrasonic on microorganisms.</P>